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I2C is the only one of the four that reads several channels together: it needs two lines, and returns a reading for each.

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Buses

You build the buses in the protocol window: each one with which channel is SDA and which is SCL. You can add and remove them.
Each bus needs two free channels: one for SDA and one for SCL. With no buses set up, the notice asks for one: “No bus yet. Add one and pick which channel is SDA and which is SCL.”
If you never touch them, the editor sets them up on its own: by the channel names, or with the first two.

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How it is read

SDA is the data and SCL the clock. Every rising edge of SCL reads one bit; with SCL high, SDA falling is START and SDA rising is STOP.

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Transfer stretches

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Errors it detects

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The summary

Each transfer is summed up with the address, the direction and the size:
  • 0x3C W · 4 B
  • 0x68 R · 2 B
  • no answer, Incomplete transfer or Clock without START when something fails

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How to start

With nothing drawn, the status box explains:
No transfer yet. With SCL high, pull SDA low for the START; after that every rising edge of SCL reads one bit of SDA.